Objective To explore simple method for cloning the products amplified with polymerase chain reaction ( PCR). 目的探讨克隆聚合酶链反应(PCR)扩增产物的简便方法。
A simple and efficient method for direct cloning of polymerase chain reaction products 一种直接高效克隆PCR产物的载体制备
A simple and rapid method was used to prepare template DNA for polymerase chain reaction, Cells and minced tissues were digested with proteinase K. 在多聚酶链反应时采用一种快速简便的模板DNA制备方法。组织块在冰浴中剪碎和匀浆,再用蛋白酶K降解;
A simple and rapid approach to the identification of commonly isolated mycobacteria Multiplex polymerase chain reaction for detection of Herpes simplex virus type 1, type 2, cytomegalovirus and varicella-zoster virus in eye bank cornea donor 一种鉴定常见分枝杆菌分离株的简单快速分子生物学方法新型分子生物学方法快速诊断眼库供体角膜病毒感染
A rapid, simple and reliable method to prepare dsRNA template for reverse transcription polymerase chain reaction is established. 应用RT-PCR技术检测草鱼出血病病毒。建立了一种快速、简易而可靠的dsRNA模板的制备方法。